Somatic hybridization in saccharomyces cerevisiae: Analysis of products of protoplast fusion
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analysis of power in the network society
اندیشمندان و صاحب نظران علوم اجتماعی بر این باورند که مرحله تازه ای در تاریخ جوامع بشری اغاز شده است. ویژگیهای این جامعه نو را می توان پدیده هایی از جمله اقتصاد اطلاعاتی جهانی ، هندسه متغیر شبکه ای، فرهنگ مجاز واقعی ، توسعه حیرت انگیز فناوری های دیجیتال، خدمات پیوسته و نیز فشردگی زمان و مکان برشمرد. از سوی دیگر قدرت به عنوان موضوع اصلی علم سیاست جایگاه مهمی در روابط انسانی دارد، قدرت و بازتولید...
15 صفحه اولSomatic hybridization in the moss Physcomitrella patens using PEG-induced protoplast fusion.
As an alternative to sexual crossing, protoplasts from two strains of moss (Physcomitrella patens) can be hybridized using polyethylene glycol (PEG). Although the efficiency is low, it requires no sophisticated apparatus. Hybrids are readily obtained using complementary auxotrophic mutants or strains with transgenic antibiotic resistance markers. It is now routine to obtain hybrids using transg...
متن کاملHigh-efficiency transformation of Saccharomyces cerevisiae cells by bacterial minicell protoplast fusion.
After a new transformation procedure, 10% of Saccharomyces cerevisiae cells were found to contain transforming DNA sequences. We used direct transfer of plasmid molecules by fusing bacterial minicell protoplasts to yeast protoplasts. Since the procedure significantly reduces the toxic effect of procaryotic protoplasm on the eucaryotic organism, it might be generally applicable in other systems ...
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De (Triticum aestivum): In vitro production and utilization of doubled haploids, p. 101–124. In: Y.P.S. Bajaj (ed.). Biotechnology in agriculture and forestry. vol. 12: Haploids in crop improvement I. Springer-Verlag, Berlin. Powell, W., P.D.S. Caligari, and J.M. Dunwell. 1986. Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor. Applied Genet. 72:458–4...
متن کاملIntroduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion.
Protoplast fusion was applied to obtain intraspecific fusion in yeast strains in order to overcome restrictions imposed by the natural mating system. Acriflavine was used to construct petite mutants from non-flocculent industrial Saccharomyces cerevisiae saké strain. UV radiation was used to construct ura- mutants from a respiratory competent and highly flocculent S. cerevisiae NCYC869 strain. ...
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ژورنال
عنوان ژورنال: Carlsberg Research Communications
سال: 1979
ISSN: 0105-1938
DOI: 10.1007/bf02906157